Why is 35S promoter used?
The 35S CaMV promoter is generally considered to be a strong constitutive promoter1 and it facilitates high level of RNA transcription in a wide variety of plants, including plants well outside the host range of the virus2.
Which problem is associated with a Caulimovirus?
|Caption||Cauliflower mosaic caulimovirus particles.|
|Copyright||Alan A. Brunt|
How do you get rid of tobacco mosaic virus?
No chemicals cure a virus-infected plant.
- Purchase virus-free plants.
- Remove all weeds since these may harbor TMV.
- Remove all crop debris from benches and the greenhouse structure.
- Set aside plants with the above symptoms and obtain a diagnosis.
- Discard infected plants.
Why are GMOs 35S?
The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). … The qualitative PCR could detect the P35S promoter in 23 unique GMO events with high specificity and sensitivity.
What primers are used to detect a GMO food?
Four primer pairs have been used for detection of GM rices. Moreover, the sucrose phosphate synthase (SPS), soy Lectin and maize invertase genes were used as rice-specific, soy-specific, and maize-specific endogenous reference genes, respectively.
What is NOS terminator?
tumefaciens) NOS terminator. This sequence has been widely used as a transcriptional terminator in synthetic gene constructs. Since A. … This kit provides a control test that detects a region of the A. tumefaciens genome that is not used in the genetic modification of plants.
What is a ubiquitin promoter?
The maize ubiquitin 1 (Ubi-1) promoter has been widely used to drive constitutive transgene expression in monocotyledonous plants. However, lack of data on its activity in individual transformed wheat lines constitutes a gap in the understanding and predictability of this promoter’s performance.
What is the drawback of using plant viruses as cloning vectors?
5. What is the drawback of using plant viruses as cloning vectors? Explanation: The main problem of using plant viruses as cloning vectors is that the vast majority of plant viruses have genomes not of DNA but RNA, and manipulations with RNA are more difficult.